Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine

doi: 10.1016/j.jmoldx.2019.07.002

Figure Lengend Snippet: Overview of Urine cfDNA Extraction Methods

Article Snippet: Norgen Urine Cell-Free Circulating DNA Purification Kit , 30–72 , 25 , Moderate , Good , Poor.

Techniques: Extraction, Purification, Selection, Hybridization, Magnetic Beads, Adsorption, DNA Purification, Clinical Proteomics, DNA Extraction

Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine

doi: 10.1016/j.jmoldx.2019.07.002

Figure Lengend Snippet: Ability to Detect Low Concentrations of Short DNA

Article Snippet: Norgen Urine Cell-Free Circulating DNA Purification Kit , 30–72 , 25 , Moderate , Good , Poor.

Techniques: Extraction, Hybridization, DNA Purification, DNA Extraction

Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine

doi: 10.1016/j.jmoldx.2019.07.002

Figure Lengend Snippet: Summary of Analytical Performance of Urine cfDNA Extraction Methods

Article Snippet: Norgen Urine Cell-Free Circulating DNA Purification Kit , 30–72 , 25 , Moderate , Good , Poor.

Techniques: Extraction, Inhibition, Hybridization, DNA Purification, DNA Extraction

Journal: Scientific Reports

Article Title: Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

doi: 10.1038/s41598-017-00578-x

Figure Lengend Snippet: Generating non-transgenic slmlo1 tomato lines resistant to powdery mildew. ( a ) The SlMlo1 locus was targeted by two sgRNAs; ( b ) T0 tomato transformants were tested for the presence of deletions using the PCR band shift assay; ( c ) Selected T0 transformants genotyped using the PCR band shift assay alongside wild type (WT); ( d ) SlMlo1 sequencing reads from selected T0 transformants; ( e ) Leaves of tomato plants inoculated with Oidium neolycopersici (5 weeks post inoculation); ( f ) PCR genotyping of the T1 generation for the presence T-DNA and the slmlo1 mutation. The agarose gels presented in panels ( b and c ) were cropped.

Article Snippet: To avoid amplification artifacts introduced during library preparation for Illumina sequencing all libraries were prepared following the TruSeq DNA PCR-free library preparation guide (Cat. No: FC-121-9006DOC) and using TruSeq DNA PCR-Free LT kit Set A (Cat. No: FC-121-3001).

Techniques: Transgenic Assay, Electrophoretic Mobility Shift Assay, Sequencing, Mutagenesis